Virus-like particles: preparation, immunogenicity and their roles as nanovaccines and drug nanocarriers
نویسندگان
چکیده
منابع مشابه
Virus-like particles as drug delivery vectors.
Virus-like particles (VLPs) assemble spontaneously during the viral cycle or in heterologous systems during expression of viral structural protein. Depending on the complexity of the VLPs, they can be obtained by expression in prokaryotic or eukaryotic expression system from the suitable recombinant vectors, or formed in cell-free conditions. Moreover, they can be built from proteins of a singl...
متن کاملPreparation and immunogenicity of influenza virus-like particles using nitrocellulose membrane filtration
PURPOSE Nitrocellulose membrane-based filtration system (NCFS) is widely used for protein concentration. In this study, we applied NCFS for production of virus-like particle (VLP) as a vaccine candidate and evaluated yield property and immunogenicity. MATERIALS AND METHODS Influenza VLPs were generated by baculovirus-insect cell protein expression system. NCFS and sucrose gradient ultracentri...
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Submit Manuscript | http://medcraveonline.com release process [1-8]. The comfort factors in using nanoscale metal particles are attained from their capability to increase the aqueous solubility of hydrophobic drugs compound, enhanced the circulation time of drugs in the blood, and repress or eliminate fast renal drugs excretion. Nevertheless, the clinical success of these agents is crucially re...
متن کاملRecombinant Hepatitis E virus like particles can function as RNA nanocarriers
BACKGROUND Assembled virus-like particles (VLPs) without genetic material, with structure similar to infectious virions, have been successfully used as vaccines. We earlier described in vitro assembly, characterisation and tissue specific receptor dependent Clathrin mediated entry of empty HEV VLPs, produced from Escherichia coli expressed HEV capsid protein (pORF2). Similar VLP's have been des...
متن کاملVirus-like particles as vaccine.
This review presents data on commercial and experimental virus-like particle (VLP) vaccines, including description of VLP vaccines against influenza. Virus-like particles are multimeric, sometimes multiprotein nanostructures assembled from viral structural proteins and are devoid of any genetic material. VLPs present repetitive high-density displays of viral surface proteins. Importantly, they ...
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ژورنال
عنوان ژورنال: Journal of Nanobiotechnology
سال: 2021
ISSN: 1477-3155
DOI: 10.1186/s12951-021-00806-7